Synergetic Loops: validating computing through laboratory work, strengthening labs with computational innovations

Next-generation algorithms play a pivotal role in accelerating advancements in the fields of synthetic biology and nanotechnology. Computational tools are particularly useful in improving sequence design of DNA origami and in modelling of the DNA folding, prior to implementation of costly laboratory experiments. However, initial experimental validation is crucial to establish the accuracy and reliability of these algorithms in real-world applications.

In this talk, I will illustrate the importance of experimental validation of a theoretical work. I will focus on a sequence selection algorithm that tries to reduce off-target interactions during folding. I will show the experimental validation of designs of DNA origami squares and triangles of varied quality. Finally, I will underscore the role of a close collaboration between computational and experimental experts, necessary to be able to test theories and guide the lab experiments.