In-vivo implementation of bioengineering circuits

Bioengineering allows us to program bacteria with predefined behaviour. We want to investigate via microfluidics how distant bacterial colonies communicate via simple diffusion. The experimental design is geared towards collecting detailed and robust data to build quantitative models aiding the design of cellular computation. Here, we created a sender-receiver system that relies on quorum-sensing as a molecular wire. We are interested in building more complex circuit designs relying on the data that will be collected from diffusion distances and optimal cell copy-number. We discuss a range of applications from signal pulser to quorum-sensing crosstalk amplification for the study of biocomputation with microscale diffusion.